ATP9B Knockout cell line (Hep G2)

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location: Home > Products > KO Cell Lines > ATP9B Knockout cell line (Hep G2)
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ATP9B Knockout cell line (Hep G2) Order

ATP9B Knockout cell line (Hep G2)
Catalog#
YKO-HT7457
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ATP9B Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ATP9B Knockout cell line (Hep G2)
Species
Human
Gene Name
ATP9B
Gene ID
374868
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript ATP9B-202)
Validation: PCR+Sanger Sequencing
ATP9B Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ATP9B gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ATP9B gene knockout ATP9B Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ATP9B Knockout cell line (Hep G2)
ATP9B Knockout cell line (Hep G2)
Catalog#
YKO-HT7457
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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  • ATP9B Knockout cell line (HeLa)

    ATP9B Knockout cell line (HeLa)

    Organism: Human
    Gene Name: ATP9B
    Gene ID: 374868
    Size: 1*10^6
    Catalog#: YKO-XN45404
  • ATP9B Knockout cell line (HCT 116)

    ATP9B Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: ATP9B
    Gene ID: 374868
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    Organism: Human
    Gene Name: ATP9B
    Gene ID: 374868
    Size: 1*10^6
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